2016 addgene plasmid Search Results


2016 addgene  (Addgene inc)
92
Addgene inc 2016 addgene
2016 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2016 addgene/product/Addgene inc
Average 92 stars, based on 1 article reviews
2016 addgene - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier
stargazin gfp lovpep sequence  (Addgene inc)
93
Addgene inc stargazin gfp lovpep sequence
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Stargazin Gfp Lovpep Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stargazin gfp lovpep sequence/product/Addgene inc
Average 93 stars, based on 1 article reviews
stargazin gfp lovpep sequence - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
2016 • gpi 2xmcherry  (Addgene inc)
92
Addgene inc 2016 • gpi 2xmcherry
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
2016 • Gpi 2xmcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2016 • gpi 2xmcherry/product/Addgene inc
Average 92 stars, based on 1 article reviews
2016 • gpi 2xmcherry - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier
addgene 65992  (Addgene inc)
93
Addgene inc addgene 65992
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Addgene 65992, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/addgene 65992/product/Addgene inc
Average 93 stars, based on 1 article reviews
addgene 65992 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
karl deisseroth rrid addgene 20298 aav5 hsyn dio hm3d gq mcherry  (Addgene inc)
96
Addgene inc karl deisseroth rrid addgene 20298 aav5 hsyn dio hm3d gq mcherry
Key Resource Table
Karl Deisseroth Rrid Addgene 20298 Aav5 Hsyn Dio Hm3d Gq Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/karl deisseroth rrid addgene 20298 aav5 hsyn dio hm3d gq mcherry/product/Addgene inc
Average 96 stars, based on 1 article reviews
karl deisseroth rrid addgene 20298 aav5 hsyn dio hm3d gq mcherry - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier
addgene 73452  (Addgene inc)
90
Addgene inc addgene 73452
Key Resource Table
Addgene 73452, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/addgene 73452/product/Addgene inc
Average 90 stars, based on 1 article reviews
addgene 73452 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
e coli dhl708  (Addgene inc)
92
Addgene inc e coli dhl708
Key Resource Table
E Coli Dhl708, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli dhl708/product/Addgene inc
Average 92 stars, based on 1 article reviews
e coli dhl708 - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier
n a scramble control shrna  (Addgene inc)
91
Addgene inc n a scramble control shrna
A) Representative images (day 30 post-injection) and B) analysis of brain metastasis-free survival (BMFS) in mice injected intracardially with PC9-pFuLT <t>or</t> <t>pFuLT-Tet-TAZ4SA-expressing</t> cells. Mice were given dox water for the duration of the study. Statistical analysis calculated by Log-rank (Mantel-Cox) test. Parental (n=13), TAZ4SA (n=17). C-D) Quantitative analysis (day 30 post-injection) of brain-metastatic index in mice injected intracardially with C) PC9-pFuLT (n=13) vs. PC9-pFuLT-Tet-TAZ4SA (n=17) or D) HCC4006-pFuLT (n=9) vs HCC4006-pFuLT-Tet-TAZ4SA (n=10) cells. Statistical analysis calculated by unpaired two-tailed t test. ** p-value < 0.01. E) Representative images of ex vivo mouse brains on day 32 post-injection with HCC4006-pFuLT or HCC4006-Tet-TAZ4SA cells. F) Diagram of iterative derivation of brain-metastatic cell lines. G) GSEA plot of TAZ4SA signature in PC9 parental vs PC9-BrM3 RNA-seq dataset. NES=normalized enrichment score. H) Venn diagram of overlapping transcripts upregulated in PC9-BrM3 and PC9-TAZ4SA cells. I) Representative images (day 42 post-injection) and J) BMFS in mice injected intracardially with PC9-BrM3 cells expressing non-target control (shNTC, n=10) or shTAZ (clone #73, n=10). K) Immunoblot to evaluate TAZ <t>shRNA</t> knockdown in PC9-BrM3 cells. Actin was used for protein loading control.
N A Scramble Control Shrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n a scramble control shrna/product/Addgene inc
Average 91 stars, based on 1 article reviews
n a scramble control shrna - by Bioz Stars, 2026-04
91/100 stars
  Buy from Supplier
paper n a recombinant dna plasmid mouse sgrna library brie in lenticrisprv2  (Addgene inc)
94
Addgene inc paper n a recombinant dna plasmid mouse sgrna library brie in lenticrisprv2
A) Representative images (day 30 post-injection) and B) analysis of brain metastasis-free survival (BMFS) in mice injected intracardially with PC9-pFuLT <t>or</t> <t>pFuLT-Tet-TAZ4SA-expressing</t> cells. Mice were given dox water for the duration of the study. Statistical analysis calculated by Log-rank (Mantel-Cox) test. Parental (n=13), TAZ4SA (n=17). C-D) Quantitative analysis (day 30 post-injection) of brain-metastatic index in mice injected intracardially with C) PC9-pFuLT (n=13) vs. PC9-pFuLT-Tet-TAZ4SA (n=17) or D) HCC4006-pFuLT (n=9) vs HCC4006-pFuLT-Tet-TAZ4SA (n=10) cells. Statistical analysis calculated by unpaired two-tailed t test. ** p-value < 0.01. E) Representative images of ex vivo mouse brains on day 32 post-injection with HCC4006-pFuLT or HCC4006-Tet-TAZ4SA cells. F) Diagram of iterative derivation of brain-metastatic cell lines. G) GSEA plot of TAZ4SA signature in PC9 parental vs PC9-BrM3 RNA-seq dataset. NES=normalized enrichment score. H) Venn diagram of overlapping transcripts upregulated in PC9-BrM3 and PC9-TAZ4SA cells. I) Representative images (day 42 post-injection) and J) BMFS in mice injected intracardially with PC9-BrM3 cells expressing non-target control (shNTC, n=10) or shTAZ (clone #73, n=10). K) Immunoblot to evaluate TAZ <t>shRNA</t> knockdown in PC9-BrM3 cells. Actin was used for protein loading control.
Paper N A Recombinant Dna Plasmid Mouse Sgrna Library Brie In Lenticrisprv2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/paper n a recombinant dna plasmid mouse sgrna library brie in lenticrisprv2/product/Addgene inc
Average 94 stars, based on 1 article reviews
paper n a recombinant dna plasmid mouse sgrna library brie in lenticrisprv2 - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
pegfp vsvg presley  (Addgene inc)
93
Addgene inc pegfp vsvg presley
A) Representative images (day 30 post-injection) and B) analysis of brain metastasis-free survival (BMFS) in mice injected intracardially with PC9-pFuLT <t>or</t> <t>pFuLT-Tet-TAZ4SA-expressing</t> cells. Mice were given dox water for the duration of the study. Statistical analysis calculated by Log-rank (Mantel-Cox) test. Parental (n=13), TAZ4SA (n=17). C-D) Quantitative analysis (day 30 post-injection) of brain-metastatic index in mice injected intracardially with C) PC9-pFuLT (n=13) vs. PC9-pFuLT-Tet-TAZ4SA (n=17) or D) HCC4006-pFuLT (n=9) vs HCC4006-pFuLT-Tet-TAZ4SA (n=10) cells. Statistical analysis calculated by unpaired two-tailed t test. ** p-value < 0.01. E) Representative images of ex vivo mouse brains on day 32 post-injection with HCC4006-pFuLT or HCC4006-Tet-TAZ4SA cells. F) Diagram of iterative derivation of brain-metastatic cell lines. G) GSEA plot of TAZ4SA signature in PC9 parental vs PC9-BrM3 RNA-seq dataset. NES=normalized enrichment score. H) Venn diagram of overlapping transcripts upregulated in PC9-BrM3 and PC9-TAZ4SA cells. I) Representative images (day 42 post-injection) and J) BMFS in mice injected intracardially with PC9-BrM3 cells expressing non-target control (shNTC, n=10) or shTAZ (clone #73, n=10). K) Immunoblot to evaluate TAZ <t>shRNA</t> knockdown in PC9-BrM3 cells. Actin was used for protein loading control.
Pegfp Vsvg Presley, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pegfp vsvg presley/product/Addgene inc
Average 93 stars, based on 1 article reviews
pegfp vsvg presley - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
thomas jessell  (Addgene inc)
91
Addgene inc thomas jessell
A) Representative images (day 30 post-injection) and B) analysis of brain metastasis-free survival (BMFS) in mice injected intracardially with PC9-pFuLT <t>or</t> <t>pFuLT-Tet-TAZ4SA-expressing</t> cells. Mice were given dox water for the duration of the study. Statistical analysis calculated by Log-rank (Mantel-Cox) test. Parental (n=13), TAZ4SA (n=17). C-D) Quantitative analysis (day 30 post-injection) of brain-metastatic index in mice injected intracardially with C) PC9-pFuLT (n=13) vs. PC9-pFuLT-Tet-TAZ4SA (n=17) or D) HCC4006-pFuLT (n=9) vs HCC4006-pFuLT-Tet-TAZ4SA (n=10) cells. Statistical analysis calculated by unpaired two-tailed t test. ** p-value < 0.01. E) Representative images of ex vivo mouse brains on day 32 post-injection with HCC4006-pFuLT or HCC4006-Tet-TAZ4SA cells. F) Diagram of iterative derivation of brain-metastatic cell lines. G) GSEA plot of TAZ4SA signature in PC9 parental vs PC9-BrM3 RNA-seq dataset. NES=normalized enrichment score. H) Venn diagram of overlapping transcripts upregulated in PC9-BrM3 and PC9-TAZ4SA cells. I) Representative images (day 42 post-injection) and J) BMFS in mice injected intracardially with PC9-BrM3 cells expressing non-target control (shNTC, n=10) or shTAZ (clone #73, n=10). K) Immunoblot to evaluate TAZ <t>shRNA</t> knockdown in PC9-BrM3 cells. Actin was used for protein loading control.
Thomas Jessell, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thomas jessell/product/Addgene inc
Average 91 stars, based on 1 article reviews
thomas jessell - by Bioz Stars, 2026-04
91/100 stars
  Buy from Supplier
2016 addgene plasmid  (Addgene inc)
95
Addgene inc 2016 addgene plasmid
A) Representative images (day 30 post-injection) and B) analysis of brain metastasis-free survival (BMFS) in mice injected intracardially with PC9-pFuLT <t>or</t> <t>pFuLT-Tet-TAZ4SA-expressing</t> cells. Mice were given dox water for the duration of the study. Statistical analysis calculated by Log-rank (Mantel-Cox) test. Parental (n=13), TAZ4SA (n=17). C-D) Quantitative analysis (day 30 post-injection) of brain-metastatic index in mice injected intracardially with C) PC9-pFuLT (n=13) vs. PC9-pFuLT-Tet-TAZ4SA (n=17) or D) HCC4006-pFuLT (n=9) vs HCC4006-pFuLT-Tet-TAZ4SA (n=10) cells. Statistical analysis calculated by unpaired two-tailed t test. ** p-value < 0.01. E) Representative images of ex vivo mouse brains on day 32 post-injection with HCC4006-pFuLT or HCC4006-Tet-TAZ4SA cells. F) Diagram of iterative derivation of brain-metastatic cell lines. G) GSEA plot of TAZ4SA signature in PC9 parental vs PC9-BrM3 RNA-seq dataset. NES=normalized enrichment score. H) Venn diagram of overlapping transcripts upregulated in PC9-BrM3 and PC9-TAZ4SA cells. I) Representative images (day 42 post-injection) and J) BMFS in mice injected intracardially with PC9-BrM3 cells expressing non-target control (shNTC, n=10) or shTAZ (clone #73, n=10). K) Immunoblot to evaluate TAZ <t>shRNA</t> knockdown in PC9-BrM3 cells. Actin was used for protein loading control.
2016 Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2016 addgene plasmid/product/Addgene inc
Average 95 stars, based on 1 article reviews
2016 addgene plasmid - by Bioz Stars, 2026-04
95/100 stars
  Buy from Supplier

Image Search Results


Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and LOVpep) injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.

Journal: The Journal of cell biology

Article Title: Mechanosensitive calcium flashes promote sustained RhoA activation during tight junction remodeling.

doi: 10.1083/jcb.202105107

Figure Lengend Snippet: Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and LOVpep) injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.

Article Snippet: Stargazin-GFP-LOVpep (referred to as GFPLOVpep) was generated by PCR amplifying the Stargazin-GFP- LOVpep sequence (Addgene plasmid #80406; Wagner and Glotzer, 2016) and ligating into the ClaI site in pCS2+.

Techniques: Injection, Activation Assay, Optogenetics, Construct, Expressing, Two Tailed Test

Key Resource Table

Journal: Neuron

Article Title: Endocannabinoid Signaling Collapse Mediates Stress-Induced Amygdalo-Cortical Strengthening

doi: 10.1016/j.neuron.2019.12.024

Figure Lengend Snippet: Key Resource Table

Article Snippet: Also see . table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit-α-cFOS Abcam 190289 RRID: AB_443538 Alexafluor 488 Donkey-α-Rabbit Invitrogen A21206 RRID: AB_221544 Rabbit-α-DAGLα Gift from Ken Mackie N/A Bacterial and Virus Strains AAV5-CMV-fDIO-Cre-mNeonGreen-wPRE This paper VB180530-1030aad rAAV2-CAG-tdTomato ( Chan et al., 2017 ) RRID: Addgene_59462 AAV5-CaMKII-ChR2(H134R)-eYFP-wPRE ( Lee et al., 2010 ) RRID: Addgene_26969 rAAV2-EF1a-mCherry-IRES-Flpo (Fenno et al., 2014) RRID: Addgene_55634 AAV5-CMV-Cre-eGFP-SV40 Gift from James M. Wilson RRID: Addgene_105545 rAAV2-pmSyn1-EBFP-Cre ( Madisen et al., 2015 ) RRID: Addgene_51507 AAV5-EF1a-DIO-ChR2(H134R)-eYFP-wPRE Gift from Karl Deisseroth RRID: Addgene_20298 AAV5-hSyn-DIO-hM3D(Gq)-mCherry ( Krashes et al., 2011 ) RRID: Addgene_44361 rAAV2-hSyn-GCaMP7f-wPRE ( Dana et al., 2016 ) RRID: Addgene_104488 Biological Samples Chemicals, Peptides, and Recombinant Proteins Rimonabant Cayman Chemical 9000484 DO34 Glixx Laboratories GLXC-09757 JZL184 Cayman Chemical 13158 CP55,940 Cayman Chemical 13608 PF3845 Cayman Chemical 13279 NESS0327 Cayman Chemical 10004184 CNO-HCl Cayman Chemical 25780 Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains CB1 f/f mice Dr. Eric Delpire N/A DAGL f/f mice Dr. Sachin Patel N/A C57 WT mice Jackson Laboratory IMSR_JAX000664 Oligonucleotides Recombinant DNA Software and Algorithms Prism 6 Graphpad www.graphpad.com pClamp10.5 Molecular Devices www.moleculardevices.com MATLAB Mathwords www.mathworks.com ANY-maze Stoelting Co www.Stoelting.com Video Freeze Med associates www.med-associates.com Other Fear Conditioning Chamber Med associates MED-VFC-SCT-M Elevated Plus Maze San Diego Instruments Elevated Zero Maze San Diego Instruments Open in a separate window Key Resource Table

Techniques: Virus, Recombinant, Software

A) Representative images (day 30 post-injection) and B) analysis of brain metastasis-free survival (BMFS) in mice injected intracardially with PC9-pFuLT or pFuLT-Tet-TAZ4SA-expressing cells. Mice were given dox water for the duration of the study. Statistical analysis calculated by Log-rank (Mantel-Cox) test. Parental (n=13), TAZ4SA (n=17). C-D) Quantitative analysis (day 30 post-injection) of brain-metastatic index in mice injected intracardially with C) PC9-pFuLT (n=13) vs. PC9-pFuLT-Tet-TAZ4SA (n=17) or D) HCC4006-pFuLT (n=9) vs HCC4006-pFuLT-Tet-TAZ4SA (n=10) cells. Statistical analysis calculated by unpaired two-tailed t test. ** p-value < 0.01. E) Representative images of ex vivo mouse brains on day 32 post-injection with HCC4006-pFuLT or HCC4006-Tet-TAZ4SA cells. F) Diagram of iterative derivation of brain-metastatic cell lines. G) GSEA plot of TAZ4SA signature in PC9 parental vs PC9-BrM3 RNA-seq dataset. NES=normalized enrichment score. H) Venn diagram of overlapping transcripts upregulated in PC9-BrM3 and PC9-TAZ4SA cells. I) Representative images (day 42 post-injection) and J) BMFS in mice injected intracardially with PC9-BrM3 cells expressing non-target control (shNTC, n=10) or shTAZ (clone #73, n=10). K) Immunoblot to evaluate TAZ shRNA knockdown in PC9-BrM3 cells. Actin was used for protein loading control.

Journal: Cell reports

Article Title: A TAZ-AXL-ABL2 feed-forward signaling axis promotes lung adenocarcinoma brain metastasis

doi: 10.1016/j.celrep.2019.11.018

Figure Lengend Snippet: A) Representative images (day 30 post-injection) and B) analysis of brain metastasis-free survival (BMFS) in mice injected intracardially with PC9-pFuLT or pFuLT-Tet-TAZ4SA-expressing cells. Mice were given dox water for the duration of the study. Statistical analysis calculated by Log-rank (Mantel-Cox) test. Parental (n=13), TAZ4SA (n=17). C-D) Quantitative analysis (day 30 post-injection) of brain-metastatic index in mice injected intracardially with C) PC9-pFuLT (n=13) vs. PC9-pFuLT-Tet-TAZ4SA (n=17) or D) HCC4006-pFuLT (n=9) vs HCC4006-pFuLT-Tet-TAZ4SA (n=10) cells. Statistical analysis calculated by unpaired two-tailed t test. ** p-value < 0.01. E) Representative images of ex vivo mouse brains on day 32 post-injection with HCC4006-pFuLT or HCC4006-Tet-TAZ4SA cells. F) Diagram of iterative derivation of brain-metastatic cell lines. G) GSEA plot of TAZ4SA signature in PC9 parental vs PC9-BrM3 RNA-seq dataset. NES=normalized enrichment score. H) Venn diagram of overlapping transcripts upregulated in PC9-BrM3 and PC9-TAZ4SA cells. I) Representative images (day 42 post-injection) and J) BMFS in mice injected intracardially with PC9-BrM3 cells expressing non-target control (shNTC, n=10) or shTAZ (clone #73, n=10). K) Immunoblot to evaluate TAZ shRNA knockdown in PC9-BrM3 cells. Actin was used for protein loading control.

Article Snippet: Recombinant DNA & Plasmids pLKO-puro Non-Target shRNA Control Sigma Mission TRC1 SHC016-1EA pLKO-puro shAXL 1040 Sigma Mission TRC1 TRCN0000001040 pLKO-puro shTAZ 70 Sigma Mission TRC1 TRCN0000019470 pLKO-puro shTAZ 71 Sigma Mission TRC1 TRCN0000019471 pLKO-puro shTAZ 73 Sigma Mission TRC1 TRCN0000019473 TetO-FUW-pgk-puro ( Chowdhury et al., 2016 ) Addgene #85747; RRID:Addgene_85747 pLVX-Tight-Puro Tet-On Vector Xaralabos Varelas, Boston University, Boston, MA, USA N/A pLVX-TP-3F-TAZ4SA Xaralabos Varelas, Boston University, Boston, MA, USA N/A Scramble control shRNA ( Gu et al, 2016 ) N/A ABL1 shRNA ( Gu et al, 2016 ) N/A ABL2 shRNA ( Gu et al, 2016 ) N/A 3XFlag pCMV5-TOPO TAZ wild type ( Varelas et al., 2008 ) Addgene #24809; RRID:Addgene_24809 3XFlag pCMV5-TOPO TAZ Y321F This paper N/A Tet-pLKO-puro ( Wiederschain et al., 2009 ) Addgene #21915; RRID:Addgene_21915 Tet-pLKO-shNTC-puro This paper N/A Tet-pLKO-shAXL-puro This paper N/A, shAXL clone TRCN0000001040 Tet-pLKO-shTAZ-puro This paper N/A, shTAZ clone TRCN0000019470 N174-MCS http://n2t.net/addgene:81061 Addgene #81061; RRID:Addgene_81061 phL1A-pcDNA3 ( Hlavin and Lemmon, 1991 ) Addgene #12307; RRID:Addgene_12307 N174-L1CAM This paper N/A pN1-eGFP Anthony Koleske, Yale University, New Haven, CT, USA N/A pN1-ABL2-eGFP WT Anthony Koleske, Yale University, New Haven, CT, USA N/A pN1-ABL2-eGFP R198K This paper N/A pN1-ABL2-eGFP K317M This paper N/A pWZL Neo Myr Flag AXL ( Boehm et al., 2007 ) Addgene #20428; RRID:Addgene_20428 pWZL Neo Myr Flag AXL Y779F This paper N/A pWZL Neo Myr Flag AXL Y821F This paper N/A pWZL Neo Myr Flag AXL Y830F This paper N/A pWZL Neo Myr Flag AXL Y866F This paper N/A Software and Algorithms Prism 6 and 8 Graphpad http://graphpad.com/scientific-software/prism ImageJ ( Schneider et al., 2012 ) http://imagej.nih.gov RStudio R Foundation for Statistical Computing http://rstudio.com CBLigand Online BBB Predictor Tool v0.90 ( Liu et al., 2014 ) http://cbligand.org Living Image Perkin Elmer http://perkinelmer.com GSEA 3.0 ( Subramanian et al., 2005 ) http://software.broadinstitute.org/gsea KM Plotter ( Gyorffy et al., 2013 ) http://kmplot.com CBioPortal v3.1.1 ( Cerami et al., 2012 ; Gao et al., 2013 ) http://cbioportal.org TrimGalore v0.4.4 Babraham Bioinformatics http://github.com/FelixKrueger/TrimGalore STAR aligner ( Dobin et al., 2013 ) http://github.com/alexdobin/STAR HTSeq-count ( Anders et al., 2015 ) http://htseq.readthedocs.io DESeq2 v2.12 ( Love et al., 2014 ) http://bioconductor.org/packages/release/bioc/html/DESeq2.html Hisat2 v2.1.0 ( Kim et al., 2019 ) http://ccb.jhu.edu/software/hisat2/index.shtml Open in a separate window KEY RESOURCES TABLE Co-immunoprecipitation assays.

Techniques: Injection, Expressing, Two Tailed Test, Ex Vivo, RNA Sequencing, Control, Western Blot, shRNA, Knockdown

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: A TAZ-AXL-ABL2 feed-forward signaling axis promotes lung adenocarcinoma brain metastasis

doi: 10.1016/j.celrep.2019.11.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Recombinant DNA & Plasmids pLKO-puro Non-Target shRNA Control Sigma Mission TRC1 SHC016-1EA pLKO-puro shAXL 1040 Sigma Mission TRC1 TRCN0000001040 pLKO-puro shTAZ 70 Sigma Mission TRC1 TRCN0000019470 pLKO-puro shTAZ 71 Sigma Mission TRC1 TRCN0000019471 pLKO-puro shTAZ 73 Sigma Mission TRC1 TRCN0000019473 TetO-FUW-pgk-puro ( Chowdhury et al., 2016 ) Addgene #85747; RRID:Addgene_85747 pLVX-Tight-Puro Tet-On Vector Xaralabos Varelas, Boston University, Boston, MA, USA N/A pLVX-TP-3F-TAZ4SA Xaralabos Varelas, Boston University, Boston, MA, USA N/A Scramble control shRNA ( Gu et al, 2016 ) N/A ABL1 shRNA ( Gu et al, 2016 ) N/A ABL2 shRNA ( Gu et al, 2016 ) N/A 3XFlag pCMV5-TOPO TAZ wild type ( Varelas et al., 2008 ) Addgene #24809; RRID:Addgene_24809 3XFlag pCMV5-TOPO TAZ Y321F This paper N/A Tet-pLKO-puro ( Wiederschain et al., 2009 ) Addgene #21915; RRID:Addgene_21915 Tet-pLKO-shNTC-puro This paper N/A Tet-pLKO-shAXL-puro This paper N/A, shAXL clone TRCN0000001040 Tet-pLKO-shTAZ-puro This paper N/A, shTAZ clone TRCN0000019470 N174-MCS http://n2t.net/addgene:81061 Addgene #81061; RRID:Addgene_81061 phL1A-pcDNA3 ( Hlavin and Lemmon, 1991 ) Addgene #12307; RRID:Addgene_12307 N174-L1CAM This paper N/A pN1-eGFP Anthony Koleske, Yale University, New Haven, CT, USA N/A pN1-ABL2-eGFP WT Anthony Koleske, Yale University, New Haven, CT, USA N/A pN1-ABL2-eGFP R198K This paper N/A pN1-ABL2-eGFP K317M This paper N/A pWZL Neo Myr Flag AXL ( Boehm et al., 2007 ) Addgene #20428; RRID:Addgene_20428 pWZL Neo Myr Flag AXL Y779F This paper N/A pWZL Neo Myr Flag AXL Y821F This paper N/A pWZL Neo Myr Flag AXL Y830F This paper N/A pWZL Neo Myr Flag AXL Y866F This paper N/A Software and Algorithms Prism 6 and 8 Graphpad http://graphpad.com/scientific-software/prism ImageJ ( Schneider et al., 2012 ) http://imagej.nih.gov RStudio R Foundation for Statistical Computing http://rstudio.com CBLigand Online BBB Predictor Tool v0.90 ( Liu et al., 2014 ) http://cbligand.org Living Image Perkin Elmer http://perkinelmer.com GSEA 3.0 ( Subramanian et al., 2005 ) http://software.broadinstitute.org/gsea KM Plotter ( Gyorffy et al., 2013 ) http://kmplot.com CBioPortal v3.1.1 ( Cerami et al., 2012 ; Gao et al., 2013 ) http://cbioportal.org TrimGalore v0.4.4 Babraham Bioinformatics http://github.com/FelixKrueger/TrimGalore STAR aligner ( Dobin et al., 2013 ) http://github.com/alexdobin/STAR HTSeq-count ( Anders et al., 2015 ) http://htseq.readthedocs.io DESeq2 v2.12 ( Love et al., 2014 ) http://bioconductor.org/packages/release/bioc/html/DESeq2.html Hisat2 v2.1.0 ( Kim et al., 2019 ) http://ccb.jhu.edu/software/hisat2/index.shtml Open in a separate window KEY RESOURCES TABLE Co-immunoprecipitation assays.

Techniques: Western Blot, Immunofluorescence, Recombinant, In Vivo, In Vitro, Mutagenesis, Caspase-Glo Assay, RNA Sequencing, shRNA, Control, Plasmid Preparation, Software